A high-resolution cytogenetic map of human chromosome 5: Localization of 206 new cosmid markers by direct R-banding fluorescence in situ hybridization

Journal Article · Thu Jul 01 04:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1310· OSTI ID:7225805

Takahashi, Ei-Ichi; Hitomi, Akitsu ^[1]; Nakamura, Yusuke ^[2]

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1. National Institute of Radiological Sciences, Chiba (Japan)
2. Cancer Institute, Tokyo (Japan)

The authors have constructed a high-resolution cytogenetic map of human chromosome 5 with 206 new cosmid clones by direct R-banding fluorescence in situ hybridization. The fluorescent signals of 206 clones were evenly distributed throughout chromosome 5, although they were sublocalized preferentially to R-positive bands. This high-resolution cytogenetic map with an average distance of nearly 1 Mb will serve as an important resource for construction of a genetic linkage map, which is essential for positional cloning of responsible genes. Moreover, these mapping data provide many useful landmarks for the construction of contig maps of chromosome 5, as required in the Human Genome Project. 15 refs., 2 figs., 1 tab.

• OSTI ID: 7225805
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 17:1; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MARKERS
CHROMOSOMES
DNA HYBRIDIZATION
GENETIC MAPPING
HUMAN CHROMOSOME 5
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING