A high-resolution cytogenetic map of human chromosome 9: Localization of 203 new cosmid markers by direct R-banding fluorescence in situ hybridization

Journal Article · Sat Jan 15 04:00:00 EST 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1073· OSTI ID:7076422

Takahashi, Ei-ichi; Hitomi, Akitsu; Itoh, Hiroko ^[1]; Koyama, Kumiko; Nakamura, Yusuke ^[2]

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1. National Institute of Radiological Sciences, Chiba (Japan)
2. Cancer Institute, Tokyo (Japan)

A high-resolution cytogenetic map of human chromosome 9 was constructed with 203 newly isolated cosmid markers by direct R-banding fluorescence in situ hybridization. The clones were localized preferentially to R-positive bands throughout chromosome 9. Although the number of clones was roughly proportional to the R-band size, many more clones (ca. 80) were mapped at the q34 region compared to its physical size. Based on these cytogenetic mapping data, one can establish physical contig maps with cosmids and yeast artificial chromosomes and a genetic linkage map essential for positional cloning of responsible genes. 13 refs., 2 figs., 1 tab.

• OSTI ID: 7076422
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 19:2; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MARKERS
CHROMOSOMES
COSMIDS
DNA HYBRIDIZATION
GENETIC MAPPING
HUMAN CHROMOSOME 9
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING