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Predominant induction of kinetochore-containing micronuclei by extracts of diesel exhaust particulates in cultured human lymphocytes

Journal Article · · Environmental and Molecular Mutagenesis; (United States)
; ; ; ;  [1];  [2]
  1. Saitama Medical School (Japan)
  2. West China Univ. of Medical Sciences, Sichuan (China)
+ Show Author Affiliations
The aneuploidy-inducing activity of extracts of diesel exhaust particulates from light duty (LD) and heavy duty (HD) engines was investigated in cultured peripheral blood lymphocytes of 8 healthy donors using the cytokinesis-block micronucleus test with the kinetochore labelling modification. A majority of the subjects tested showed a significant kinetochore-positive micronucleus induction after treatment with the highest dose (150 [mu]g/ml) of LD extract, although some subjects also showed induction of kinetochore-negative micronuclei. Only one subject had significantly increased numbers of kinetochore-positive micronuclei at a dose of 400 [mu]g/ml of HD extract. These results suggest that diesel extract, at least LD extract, possesses the ability to induce whole chromosome loss (aneuploidy) preferentially, although there are also chromosome breaks. 21 refs., 2 figs., 3 tabs.
OSTI ID:
7225137
Journal Information:
Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 23:1; ISSN 0893-6692; ISSN EMMUEG
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEUPLOIDY
ANIMAL CELLS
CHROMOSOMAL ABERRATIONS
DIESEL FUELS
DISTILLATES
ENERGY SOURCES
EXHAUST GASES
FLUIDS
FOSSIL FUELS
FUELS
GAS OILS
GASEOUS WASTES
GASES
INDUCTION
LIQUID FUELS
MUTATIONS
PARTICLES
PARTICULATES
PETROLEUM
PETROLEUM DISTILLATES
PETROLEUM FRACTIONS
PETROLEUM PRODUCTS
PLOIDY
TOXICITY
WASTES