Centromere detection in vinblastine- and radiation-induced micronuclei of cytokinesis-blocked mouse cells by using in situ hybridization with a mouse gamma (major) satellite DNA probe

Journal Article · Wed Jan 01 04:00:00 EST 1992 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850190102· OSTI ID:5679232

Salassidis, K; Huber, R; Zitzelsberger, H; Bauchinger, M ^[1]

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1. Inst. fuer Strahlenbiologie, Neuherberg (West Germany)

Non-isotopic in situ hybridization using a mouse gamma (major) satellite probe DNA was applied to detect centromeres in micronuclei, which were induced in vitro mouse liver cells by ionizing radiation and by vinblastine sulfate. In a cytokinesis-blocked micronucleus assay a dose-dependent induction of micronuclei was found for both agents. After vinblastine exposure the observed micronuclei showed centromere-positive hybridization signals in an order of magnitude of 70-91%, but after radiation exposure the magnitude was only 10-20%. Since the in situ hybridization technique detects centromeric DNA directly, it can be used in a cytokinesis-blocked micronucleus assay for a rapid and reliable discrimination between aneuploidy-inducing and clastogenic agents.

• OSTI ID: 5679232
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 19:1; ISSN EMMUE; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEUPLOIDY
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CELL NUCLEI
DNA HYBRIDIZATION
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GENE MUTATIONS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HYBRIDIZATION
IONIZING RADIATIONS
LIVER CELLS
MUTATIONS
PLOIDY
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
SOMATIC CELLS