Slow phase hemolysis in hypotonic electrolyte solutions
When a population of erythrocytes is partially hemolyzed the time course of hemolysis can be divided into a fast phase and a slow phase. The slow phase occurs with both rapid and gradual addition of the hypotonic medium (rapid and gradual hemolysis). There is no difference in the osmotic fragility of erythrocytes remaining at 60 minutes after rapid or gradual hemolysis. Erythrocytes near their critical hemolytic volume have an equimolar ouabain-insensitive sodium-potassium exchange. Critical non-hemolytic swelling with resulting stress on the membrane appears requisite to slow phase hemolysis since more non-penetrant sucrose is required to prevent slow phase lysis rather than that which would be predicted from the intracellular colloid osmotic pressure due to hemoglobin. Sucrose protection from slow phase hemolysis thus depends not only on counter-balancing the colloid osmotic pressure, but also removal of sufficient intracellular water to prevent critical membrane strain. This model is consistent with that proposed by Katchalsky. Irreversible membrane changes associated with hypotonic stress manifested by persistent stomatocytic shape change and membrane wrinkling on return of cells to isotonicity appear to be due to critical changes in membrane components. Such cells, having normal indices and specific gravity, are less deformable than control cells in 2.8 ..mu..m pore size polycarbonate filters.
- Research Organization:
- Univ. of Rochester, NY
- OSTI ID:
- 7216577
- Journal Information:
- J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 85:1; ISSN JCLLA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKALI METALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARDIOTONICS
CELL CONSTITUENTS
CELL MEMBRANES
DISACCHARIDES
DISEASES
DISPERSIONS
DRUGS
ELECTROLYTES
ELEMENTS
ERYTHROCYTES
GLYCOSIDES
HEMOLYSIS
LYSIS
MEMBRANES
METALS
MIXTURES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
OUABAIN
PATHOLOGICAL CHANGES
POTASSIUM
SACCHARIDES
SACCHAROSE
SODIUM
SOLUTIONS
STEROIDS
STROPHANTIN