Radiosensitivity of T and B lymphocytes. IV. Effect of whole body irradiation upon various lymphoid tissues and numbers of recirculating lymphocytes. [Mice]

Journal Article · Fri Apr 01 04:00:00 EST 1977 · J. Immunol.; (United States)

OSTI ID:7215288

Anderson, R E; Olson, G B; Autry, J R; Howarth, J L; Troup, G M; Bartels, P H

Groups of 10-week-old-female CBA/J mice were exposed in whole body fashion to 0, 5, 50, and 500 rads and sacrificed in serial fashion 1, 3, 5, 7, 9, 15, and 30 days after irradiation for morphologic evaluation of thymus, spleen, lymph node, and Peyer's patch, and assessment of the relative numbers of thymus-derived (T) and bone marrow-derived (B) cells in these tissues. The absolute and relative numbers of recirculating T and B cells mobilizable by thoracic duct cannulation were also determined and compared with similar determinations with respect to peripheral blood lymphocytes. B cell depletion occurred more quickly and was more pronounced in spleen and lymph node than T cell depletion at all three exposure doses. Depletion of T and B cells was roughly equal in peripheral blood and thoracic duct lymph. When present, regeneration of the T cell component occurred more rapidly than did B cell restoration. The latter often was incomplete at the time of the final sacrifice (day 30). PHA-responsive and Con A-responsive cells also appeared to differ with respect to the kinetics of cell death after whole body irradiation.

• Research Organization: Univ. of New Mexico, Albuquerque
• OSTI ID: 7215288
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 118:4; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
BONE MARROW CELLS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
EXTERNAL IRRADIATION
HEMAGGLUTININS
HEMATOPOIETIC SYSTEM
IRRADIATION
LEUKOCYTES
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MICE
MITOGENS
MUCOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RADIOSENSITIVITY
RODENTS
SACCHARIDES
SOMATIC CELLS
SPLEEN
THYMOCYTES
THYMUS
TISSUES
VERTEBRATES
WHOLE-BODY IRRADIATION