Simultaneous radioimmunoassay of thyrotropin and thyroxine in human serum. [/sup 125/I and /sup 131/I tracer techniques]
Journal Article
·
· Clin. Chem.; (United States)
OSTI ID:7211832
The importance of early diagnosis and treatment of congenital hypothyroidism has been well established, and several screening programs have been undertaken to detect neonates with this disorder by measurement of concentrations of thyrotropin or thyroxine in the serum. However, measurement of either hormone alone may fail to identify all affected patients. Accordingly, we have established a simulatneous double-antibody, dual-isotope radioimmunoassay for both. Sensitivity, slope, analytical recovery, and precision characteristics of the simultaneous assay do not differ from those of each assay performed separately. Values for the two analyses in the single and simultaneous assays correlate well (r = 0.951 for thyroxine, 0.983 for thyrotropin). This assay system permits determination of both hormones within 72 h after specimen collection and thus should allow more rapid evaluation, diagnosis, and treatment of infants with congenital hypothyroidism.
- Research Organization:
- All Children's Hospital, St. Petersburg, FL
- OSTI ID:
- 7211832
- Journal Information:
- Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 23:9; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Assessment of a method for measuring serum thyroxine by radioimmunoassay, with use of polyethylene glycol precipitation. [/sup 125/I tracer technique]
Measuring thyroxine and thyrotropin simultaneously in a dried blood sample on filter paper, to screen for neonatal hypothroidism
Determination of free thyroxine in serum by radioimmunoassay. [/sup 125/I and /sup 131/I tracer techniques]
Journal Article
·
Fri Jul 01 00:00:00 EDT 1977
· Clin. Chem.; (United States)
·
OSTI ID:7323965
Measuring thyroxine and thyrotropin simultaneously in a dried blood sample on filter paper, to screen for neonatal hypothroidism
Journal Article
·
Thu May 01 00:00:00 EDT 1980
· Clin. Chem. (Winston-Salem, N.C.); (United States)
·
OSTI ID:6058101
Determination of free thyroxine in serum by radioimmunoassay. [/sup 125/I and /sup 131/I tracer techniques]
Journal Article
·
Thu Sep 01 00:00:00 EDT 1977
· Clin. Chem.; (United States)
·
OSTI ID:7285220
Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD SERUM
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DOUBLE LABELLING
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
NEONATES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
THYROID HORMONES
THYROXINE
TRACER TECHNIQUES
TSH
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD SERUM
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DOUBLE LABELLING
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
NEONATES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
THYROID HORMONES
THYROXINE
TRACER TECHNIQUES
TSH