Partial amino acid sequence of brain actin and its homology with muscle actin

Journal Article · Tue Dec 27 04:00:00 EST 1977 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00645a025· OSTI ID:7208236

Lu, R C; Elzinga, M

Actin was purified from calf brains by chromatography on DEAE-Sephadex and hydroxylapatite. The protein was then subjected to amino acid sequence analysis by isolating and sequencing its cyanogen bromide peptides. CB-1, 3, 4, 5, 6, 9, 10, and 12 correspond to equivalent segments of rabbit skeletal muscle actin, while substitutions involving methionines give rise to some new peptides. The region that corresponds to CB-13 in muscle actin becomes two peptides in the brain protein because of a Leu ..-->.. Met replacement at position 16, while Met ..-->.. Leu substitutions at positions 176 and 298 give rise to two larger peptides, CB-15+7 and CB-8 + 2, which correspond to muscle actin CB-15 fused with CB-7 and CB-8 fused with CB-2, respectively. The peptides that have been isolated from brain actin contain 267 of the 374 residues in actin, of which 157 have been unequivocally identified. When the data are compared with those for rabbit skeletal muscle actin, 11 replacements are seen; thus the two actins differ at about 7 percent of the positions examined.

• Research Organization: Boston Biomedical Research Inst.
• OSTI ID: 7208236
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 16:26; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550300 -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ACTIN
AMINO ACIDS
ANIMALS
BODY
BRAIN
CALVES
CARBOXYLIC ACIDS
CATTLE
CENTRAL NERVOUS SYSTEM
COMPARATIVE EVALUATIONS
DOMESTIC ANIMALS
MAMMALS
MOLECULAR STRUCTURE
MUSCLES
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RABBITS
RUMINANTS
VERTEBRATES