Investigations into the use of radiolabeled monoclonal antibodies for selective cell labeling in whole blood; Final report
Technical Report
·
OSTI ID:720368
Our previous work in which 17 monoclonal antibodies (MAbs), 7 for human platelets and 10 for human neutrophils, were evaluated, led us to identifying one MAb with excellent merit. This antibody, Anti Stage Specific Embryonic Antigen-1 (SSEA-1 or MCA 480), is highly specific (1.6 {times} 10{sup {minus}11}M) for human neutrophils (PMNs). It has a high afinity (80 {plus_minus} 3%), 5.1 {times} 10{sup 5} antigenic determinants per PMN and produces consistent and reproducible results. Techniques have been developed to label this MAb with In-111 as well as Tc-99m in excellent yields. A commercial market analysis revealed that annually in the United States alone there are as many as 1,880,000 patients who could be given Tc-99m PMN specific antibody for imaging abscesses. This technique is expected to provide the diagnostic results in much shorter period of time (3 hr vs 24 hr), at lower cost and at lower radiation risk than the currently used nuclear medicine techniques. Our aim is to further evaluated, Tc-99m or In-111-anti-SSEA-1, in patients clinically proven to have harboring abscesses. During the past 9 months we obtained and evaluated two more antibodies (P201, an IqG, and P203, and IgM) specific for platelets. However the major thrust of our work during this period (March 88-Dec. 88) was to generate additional data so that we would write an IND application and obtain the FDA approval for using anti-SSEA-1 in patients for imaging abscesses. This included several steps, the results of which have been summarized below. The IND has been submitted and the early FDA questions have been answered.
- Research Organization:
- Thomas Jefferson Univ., Philadelphia, PA (United States). Hospital
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-85ER60295
- OSTI ID:
- 720368
- Report Number(s):
- DOE/ER/60295--4; ON: TI90020727
- Country of Publication:
- United States
- Language:
- English
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