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Investigations into the use of radiolabeled monoclonal antibodies for selective cell labeling in whole blood: Progress report, January 1986-December 1986

Technical Report ·
OSTI ID:6782611
Techniques to effectively labeling blood cell specific monoclonal antibodies (MAbs), with In-111 using bifunctional chelating agents such as the cyclic or mixed anhydride of diethylenetriaminepentaacetic acid have been developed. Conditions for the optimal labeling of blood cells are reported. The specificity (Kd values) of In-111 labeled MAbs for the cell specific antigens are reported. In-111-MAb labeled canine platelets were used to image vascular thrombi. Under the best experimental conditions approximately 70% of the radiolabeled MAbs get incorporated into isolated blood cells. However, only about 35% of the added MAb-associated radioactivity would incorporate into the desired type of blood cells in whole blood. The chemical nature of the radioactivity that does not bind to the cells was investigated. 9 figs., 6 tabs.
Research Organization:
Thomas Jefferson Univ., Philadelphia, PA (USA). Hospital
DOE Contract Number:
FG02-85ER60295
OSTI ID:
6782611
Report Number(s):
DOE/ER/60295-2; ON: DE87005410
Country of Publication:
United States
Language:
English

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(Investigations into the use of radiolabeled monoclonal antibodies for selective cell labeling in whole blood):
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Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400700 -- Radiochemistry & Nuclear Chemistry
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHEMISTRY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DOGS
DRUGS
DTPA
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ERYTHROCYTES
GLOBULINS
GLOBULINS-BETA
HOURS LIVING RADIOISOTOPES
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
LEUKOCYTES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
METALLOPROTEINS
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RABBITS
RADIOCHEMISTRY
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
SEPARATION PROCESSES
TECHNETIUM 99
TECHNETIUM ISOTOPES
TRANSFERRIN
VERTEBRATES
YEARS LIVING RADIOISOTOPES