Evaluation of I-123 and In-111 labeled anti-platelet monoclonal antibody for the scintigraphic localization of in vivo thrombi
Development of a method to selectively label platelets in whole blood in vitro or in vivo is a highly desirable goal. The authors have investigated the labeling with I-123, I-131, and In-111 of an IgG/sub 1/ monoclonal antibody, 7E3 (MAb) that specifically inhibits the interaction of dog platelets with fibrinogen-coated beads and blocks ADP-induced aggregation of dog platelets. The MAb, typically 100 ..mu..g, was radioiodinated using the chloramine T method. Following a G-25 or P-6 column purification, labeling yields of approx. =70% were achieved (2 I/MAb). The MAb-DTPA conjugate was labeled with In-111 in >80% yields with a specific activity of 10-30 ..mu..Ci/..mu..g (approx. =0.1 In/MAb). Retention of inhibiting activity in the fibrinogen-coated bead assay was excellent for both I-123 and In-111 labeled 7E3. In dogs, the blood pool activity persisted for up to 24 hr and some urinary excretion (In-111) and deiodination (I-123) were observed in vivo. When In-111-MAb was incubated with whole blood (0.13 ..mu..g/ml) and the mixture then clotted with thrombin (l..mu../ml), 68% of the radioactivity remained with the clot despite repeated washing. Preliminary in vivo data in dogs suggest that the label becomes localized at the site of vascular injury and/or thrombus formation. The authors conclude that radiolabeled monoclonal anti-platelet antibodies may show promise for imaging vascular lesions and thrombi.
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 6932277
- Report Number(s):
- CONF-840619-
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 25:5; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
CARDIOVASCULAR DISEASES
COAGULANTS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DISEASES
DOGS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FIBRINOGEN
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
HOURS LIVING RADIOISOTOPES
IMAGE PROCESSING
IMMUNOGLOBULINS
IN VITRO
IN VIVO
INDIUM 111
INDIUM ISOTOPES
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 123
IODINE 131
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROCESSING
PROTEINS
RADIOISOTOPE SCANNING
RADIOISOTOPES
SCINTISCANNING
THROMBOSIS
USES
VASCULAR DISEASES
VERTEBRATES