Thrombus radioimmunoscintigraphy: an approach using monoclonal antiplatelet antibody
An approach to thrombus imaging using a monoclonal antiplatelet antibody labeled with In or STI is described. The antibody (7E3) prepared against human platelets inhibits the interaction between fibrinogen-coated beads and both human and dog platelets. 7E3 is an IgG1 that binds to the complexed glycoprotein IIb/IIIa. Ninety percent of a tracer dose of radiolabeled 7E3 binds to human platelets and 50% binds to dog platelets. In vitro studies showed that virtually all of the platelet-bound radioactivity becomes incorporated into clots formed by adding thrombin to whole blood. 7E3 was labeled with In by the cyclic anhydride diethylenetriaminepentaacetic acid method or by radioiodination with STI. With el camera, visualization of venous and arterial thrombi as well as sites of initial injury without visible thrombi, could be observed 1-1.5 hr after injection. There was no need for delayed imaging because of the fast clearance of radioactivity from the circulation nor was there need for blood pool subtraction. Two to 10-hr thrombi could be imaged but 48-hr thrombi were not detectable with this method. No change in platelet counts before and after the injection of labeled 7E3 nor increased bleeding tendency occurred. The advantages of this method are a shorter preimaging preparation time, faster visualization after injection, and no need for delayed imaging or subtraction techniques. For these reasons human investigations seem to be warranted.
- Research Organization:
- State Univ. of New York, Stony Brook
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 6289533
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 82:10; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARDIOVASCULAR DISEASES
CHEMICAL PREPARATION
COUNTING TECHNIQUES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EMBOLI
HOURS LIVING RADIOISOTOPES
IMMUNOLOGY
INDIUM 122
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 123
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MATERIALS
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIONUCLIDE KINETICS
RADIOPHARMACEUTICALS
SCINTISCANNING
SECONDS LIVING RADIOISOTOPES
SYNTHESIS
THROMBOSIS
TRACER TECHNIQUES
VASCULAR DISEASES