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Radioimmunoimaging of experimental thrombi in dogs using Tc-99m labeled monoclonal antibody fragments (MAPab-F(ab')/sub 2/) reactive with human platelets

Conference · · J. Nucl. Med.; (United States)
OSTI ID:6795022
; ; ; ; ; ; ;

Radioimmunoimaging of thrombi could have great clinical value in the management of coronary artery and thromboembolic disease. In-111-oxine-labeled platelets currently used require platelet isolation, delayed imaging, background subtraction and there is also potential for damaging or contaminating platelets during labeling. Murine monoclonal antibody (IgG/sub 2/a) fragments directed against human platelet membrane components (cross-reactive with dog platelets) were labeled with Tc-99m and repurified from ''kits''. After radiolabeling, 91.5-93.3% of the Tc-99m was antibody-associated. The preparations retained immunoreactivity, as determined by the ratio of cell to plasma-associated radioactivity (ratios 54.7-63.8). Tc-99m-MAPAb-F(ab')/sub 2/ were injected i.v. into dogs with thrombi produced in peripheral and pulmonary veins and arteries. About 50% of the radioactivity was cleared from the blood in 3-6 min. and 18-24% was excreted in the urine within 3 hrs. The thrombi were consistently and easily visible within 1-3 hrs. with no need for blood pool subtraction. In some cases, intimal damage along the path of catheters was seen. No adverse side effects were observed. The advantages of this method are: short and simple preparation, no need for blood pool subtraction and early visualization of thrombi. Human studies are warranted to determine its clinical efficacy.

Research Organization:
Brookhaven National Lab., Upton, NY
OSTI ID:
6795022
Report Number(s):
CONF-850611-
Journal Information:
J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 26:5; ISSN JNMEA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOMEDICAL RADIOGRAPHY
BLOOD COAGULATION
BLOOD VESSELS
BODY
CARDIOGRAPHY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CAT SCANNING
CHEMICAL PREPARATION
COMPUTERIZED TOMOGRAPHY
CORONARIES
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DISEASES
DISTRIBUTION
DOGS
DRUGS
DYNAMIC FUNCTION STUDIES
EMBOLI
HEART
HOURS LIVING RADIOISOTOPES
IMAGES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
MAMMALS
MEDICAL SUPPLIES
MEDICINE
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PROSTHESES
RADIOASSAY
RADIOCARDIOGRAPHY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RADIOLOGY
RADIOPHARMACEUTICALS
SYNTHESIS
TECHNETIUM 99
TECHNETIUM ISOTOPES
THROMBOSIS
TISSUE DISTRIBUTION
TOMOGRAPHY
TRACER TECHNIQUES
USES
VASCULAR DISEASES
VERTEBRATES
YEARS LIVING RADIOISOTOPES