Gas chromatographic analysis of methyl formate and application in methanol poisoning cases
- Dalhousie Univ., Halifax, Nova Scotia (Canada)
A modified headspace gas chromatographic method for analysis of formate in biological fluids is described. Serum or whole blood specimens were methylated in the presence of concentrated sulfuric acid and sodium propionate (internal standard) dissolved in methanol. Derivatization was performed at 35{degree}C for 30 min before injection (0.5 mL headspace) onto a Hallcomid-Carbowax packed GLC column. Using serum or aqueous standards, the method was linear from 5 to 100 mg/dL. The limit of detection was 2.5 mg/dL. Day-to-day precision was less than 5% (CV) at 54 mg/dL formate. Formate and methanol were analyzed in 3 methanol poisonings, two of which were fatal. Formate analysis is considered important in any patient with suspected methanol poisoning who presents for medical assistance with metabolic acidosis. The extent of ocular toxicity correlates better with formate concentration than with methanol concentration.
- OSTI ID:
- 7201851
- Journal Information:
- Journal of Analytical Toxicology; (USA), Journal Name: Journal of Analytical Toxicology; (USA) Vol. 13:2; ISSN 0146-4760; ISSN JATOD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400102* -- Chemical & Spectral Procedures
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CARBOXYLIC ACID SALTS
CHROMATOGRAPHY
FORMATES
GAS CHROMATOGRAPHY
HAZARDS
HEALTH HAZARDS
HYDROXY COMPOUNDS
MATERIALS
METHANOL
ORGANIC COMPOUNDS
PATIENTS
SEPARATION PROCESSES