Sublocalization of 21 chromosome 18-specific microsatellite markers
- Thomas Jefferson Univ., Philadelphia, PA (United States)
Few polymorphic markers have been mapped to chromosome 18 compared with other human chromosomes. Recently, a concerted effort has been made to generate a microsatellite genetic linkage map of chromosome 18 using markers that have also been physically mapped to the chromosome. However, distances between some of the markers in this map are as large as 28.5 cM. Thus, additional markers are needed to complement and extend this genetic map. Twenty-one additional microsatellite markers have been mapped to chromosome 18 by Weissenbach et al. Although the markers appear to be spaced at regular intervals along the chromosome based on recombination frequencies, the physical locations of these markers have not been determined. To determine the physical location of the 21 markers on chromosome 18, the markers were regionally mapped using a panel of 20 somatic cell hybrids that contain deletions or translocations involving chromosome 18. The localizations of the markers were determined using genomic DNA from the cell hybrids in PCR experiments. 9 refs., 1 fig.
- OSTI ID:
- 7200949
- Journal Information:
- Genomics; (United States), Vol. 18:1; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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