Identification and localization of microsatellite markers covering human chromosome 18

Journal Article · Tue Dec 01 00:00:00 EST 1992 · Genomics; (United States)

DOI:https://doi.org/10.1016/S0888-7543(05)80136-3· OSTI ID:6758453

Rojas, K; Kurtz, A; Feder, M; Mewar, R; Overhauser, J ^[1]; Straub, R E; Gilliam, T C ^[2]

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1. Thomas Jefferson Univ., Philadelphia, PA (United States)
2. Columbia Univ., New York, NY (United States)

To generate microsatellite markers from chromosome 18, the authors have cytogenetically localized a large number of [lambda] phage using a deletion mapping panel of somatic cell hybrids. Here they describe the identification of 65 new CA-repeat-containing phage and the localization of five markers developed in other laboratories. This approach allows the selection of a subset of markers that are well spaced across the chromosome and can be developed as genetic markers. The use of PCR-based markers should allow for the rapid genomic screening of disease genes on chromosome 18. 11 refs., 1 fig., 1 tab.

• OSTI ID: 6758453
• Journal Information: Genomics; (United States), Vol. 14:4; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MARKERS
GENETIC MAPPING
HUMAN CHROMOSOME 18
CHROMOSOMES
HUMAN CHROMOSOMES
MAPPING
550400* - Genetics