Twenty-one polymorphic markers from human chromosome 12 for integration of genetic and physical maps
Journal Article
·
· Genomics; (United States)
- Albert Einstein College of Medicine, Bronx, NY (United States)
- Yale Univ. Medical School, New Haven, CT (United States)
Twenty-one physically mapped, polymorphic markers have been developed from a chromosome 12-specific cosmid library. The markers consist of CA repeat-containing sequence-tagged sites (STSs) derived from cosmid clones mapped by fluorescence in situ hybridization (FISH). Three methods for determining the sequence flanking CA microsatellites were used, including one using degenerate primer sets for direct sequence analysis. Oligonucleotide primer pairs suitable for use in polymerase chain reaction (PCR) were selected from the sequences flanking the CA microsatellite and were tested for their ability to generate unique PCR products. The informativeness of these STSs as genetic markers was determined by typing 10 unrelated individuals who are part of the Centre d'Etude du Polymorphisme Humaine (EPH) pedigrees. Eleven of the 21 FISH-mapped, polymorphic STSs are heterozygous in 7 or more of the individuals tested. Since these markers are derived from physically mapped cosmids, genetic linkage analysis with them will facilitate the integration of the developing physical and genetic maps of chromosome 12. 29 refs., 4 figs., 2 tabs.
- OSTI ID:
- 7200910
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 19:2; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
Similar Records
Phosphoenolpyruvate carboxykinase (GTP): Characterization of the human PCK1 gene and localization distal to MODY on chromosome 20
Assignment of 112 microsatellite markers to 23 chromosome 11 subregions delineated by somatic hybrids: Comparison with the genetic map
VNTR and microsatellite polymorphisms within the subtelomeric region of 7q
Journal Article
·
Tue Jun 01 00:00:00 EDT 1993
· Genomics; (United States)
·
OSTI ID:7077102
Assignment of 112 microsatellite markers to 23 chromosome 11 subregions delineated by somatic hybrids: Comparison with the genetic map
Journal Article
·
Sun May 15 00:00:00 EDT 1994
· Genomics; (United States)
·
OSTI ID:6872327
VNTR and microsatellite polymorphisms within the subtelomeric region of 7q
Journal Article
·
Sun Aug 01 00:00:00 EDT 1993
· American Journal of Human Genetics; (United States)
·
OSTI ID:5957324