Large-scale isolation of intact vacuoles and isolation of chloroplasts from protoplasts of mature plant tissues
Journal Article
·
· Science; (United States)
Intact plant vacuoles were prepared in large numbers (10/sup 6/) from protoplasts of mature leaves, flower petals, stems, pedicels, filaments, styles, and young fruits. Treatment of protoplasts with 0.2 molar K/sub 2/HPO/sub 4/-HCl, pH 8, with slow stirring resulted in gentle osmotic rupture of the protoplasts and release of intact vacuoles. Particulate components of the protoplast, less the vacuole, were largely shed as an aggregate, which was removed by filtration. Vacuoles were recovered from the filtrate by low-speed centrifugation. The general procedure was also used to isolate chloroplasts with a high degree of integrity and excellent photochemical activity. (auth)
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 7199543
- Journal Information:
- Science; (United States), Journal Name: Science; (United States) Vol. 190:4221; ISSN SCIEA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Content and vacuole extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts
Localization of acid hydrolases in protoplasts. Examination of the proposed lysosomal function of the mature vacuole
Vacuole/extravacuole distribution of soluble protease in Hippeastrum petal and Triticum leaf protoplasts
Journal Article
·
Sun Jul 01 00:00:00 EDT 1979
· Plant Physiol.; (United States)
·
OSTI ID:5558471
Localization of acid hydrolases in protoplasts. Examination of the proposed lysosomal function of the mature vacuole
Journal Article
·
Wed Jun 01 00:00:00 EDT 1977
· Plant Physiol.; (United States)
·
OSTI ID:7218593
Vacuole/extravacuole distribution of soluble protease in Hippeastrum petal and Triticum leaf protoplasts
Journal Article
·
Sat Oct 31 23:00:00 EST 1981
· Plant Physiol.; (United States)
·
OSTI ID:5842916