Vacuole/extravacuole distribution of soluble protease in Hippeastrum petal and Triticum leaf protoplasts
Journal Article
·
· Plant Physiol.; (United States)
The subcellular distribution of soluble protease in anthesis-stage, anthocyanin-containing Hippeastrum cv. Dutch Red Hybrid petal protoplasts has been reevaluated and that of Triticum aestivum L. var. Red Coat leaf protoplasts determined using /sup 125/I-fibrin as a protease substrate and improved methods for protoplast and vacuole volume estimation. Results indicate that about 20% of the Hippeastrum petal-soluble protease and about 90% of the wheat leaf-soluble protease can be assigned to the vacuole. Protoplast isolation enzyme labeled with /sup 125/I has been used to assess the efficiency of removing isolation enzyme from protoplasts by repeated washing and by separation of protoplasts from debris using density centrifugation. Results of these studies suggest that protoplasts prepared by both methods retain low levels of isolation enzyme. However, when protoplasts prepared by either method were lysed with washing medium lacking osmoticum, little isolation enzyme contaminated the lysates.
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 5842916
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 68:5; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
CELL CONSTITUENTS
CENTRIFUGATION
COAGULANTS
DATA
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
FIBRIN
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MEASURING METHODS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PLANT CELLS
PROTEINS
RADIOISOTOPES
SCLEROPROTEINS
SEPARATION PROCESSES
SUBSTRATES
TRACER TECHNIQUES
ULTRACENTRIFUGATION
UPTAKE
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
CELL CONSTITUENTS
CENTRIFUGATION
COAGULANTS
DATA
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
FIBRIN
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MEASURING METHODS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PLANT CELLS
PROTEINS
RADIOISOTOPES
SCLEROPROTEINS
SEPARATION PROCESSES
SUBSTRATES
TRACER TECHNIQUES
ULTRACENTRIFUGATION
UPTAKE