High-speed chromosome sorting

Journal Article · Fri Oct 16 00:00:00 EDT 1987 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.2443974· OSTI ID:7196463

Gray, J W; Dean, P N; Fuscoe, J C; Peters, D C; Trask, B J; van den Engh, G J; van Dilla, M A

Dual-beam high-speed sorting has been developed to facilitate purification of chromosomes based on DNA staining with the fluorescent dyes Hoechst 33258 and chromomycin A3. Approximately 200 chromosomes per second of two types can be sorted from a suspension of chromosome isolated from human lymphoblasts while fluorescent objects (chromosomes, debris fragments, chromosome clumps, and nuclei) are processed at the rate of about 20,000 per second. This sorting rate is approximately ten times that possible with conventional sorters. Chromosomes of a single type can be sorted with a purity of about 90 percent. DNA from the sorted chromosomes is suitable for construction of recombinant DNA libraries and for gene mapping.

• Research Organization: Lawrence Livermore National Lab., CA (USA)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 7196463
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 238:4825; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOME SORTING
DNA
DYES
FLUORESCENCE
GENETIC MAPPING
LUMINESCENCE
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RECOMBINANT DNA
STAINS