Bacterial characterization by flow cytometry

Journal Article · Fri May 06 04:00:00 EDT 1983 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.6188215· OSTI ID:5714377

Van Dilla, M A ^[1]; Langlois, R G; Pinkel, D; Yajko, D; Hadley, W K

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1. Lawrence Livermore National Lab., CA

Bacteria were analyzed in a dual-beam flow cytometer after double staining with the fluorescent dyes chromomycin A3 and Hoechst 33258, which bind preferentialy to DNA that is rich in guanine-cytosine and adenine-thymine, respectively. The measurements were indicative of the cellular DNA content and base composition, cell concentration, and proliferative state of the population. The ratio of the chromomycin A3 signal to the Hoechst 33258 signal increased with the guanine-cytosine content of the cellular DNA for the six cultured species measured, following expectation. Bacteria in urine from patients with urinary tract infections were characterized without interference from host cell DNA, debris, or other particulates.

• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5714377
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 220; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ADENINES
AMINES
ANTIMETABOLITES
AZINES
BACTERIA
BACTERIAL DISEASES
BIOCHEMISTRY
CELL CONSTITUENTS
CELL FLOW SYSTEMS
CHEMISTRY
CYTOCHEMISTRY
CYTOLOGICAL TECHNIQUES
CYTOSINE
DIAGNOSTIC USES
DISEASES
DNA
DRUGS
DYES
FLUORESCENCE
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFECTIOUS DISEASES
LUMINESCENCE
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PATIENTS
PURINES
PYRIMIDINES
THYMINE
URACILS
UROGENITAL SYSTEM DISEASES
USES