Choice of infusion-sampling mode for tracer studies of free fatty acid metabolism

Journal Article · Sun May 01 04:00:00 EDT 1988 · Am. J. Physiol.; (United States)

OSTI ID:7196075

Jensen, M D; Rogers, P J; Ellman, M G; Miles, J M

To determine the preferred infusion-sampling mode for isotopic studies of free fatty acid (FFA) metabolism, tracer ((/sup 14/C)palmitate) was infused into the left ventricle of five anesthetized dogs, and tracee ((/sup 3/H)palmitate) was infused into three separate peripheral veins of each dog. The (/sup 14/C)palmitate specific activity (SA) was lower in mixed venous than arterial blood, and (/sup 3/H)palmitate SA was equal in both sites. The actual infusion rate of (/sup 3/H)palmitate (2.15 +/- 0.31 X 10(5) disintegrations/min (dpm).kg-1.min-1) could be accurately predicted (2.14 +/- 0.32 X 10(5) dpm.kg-1.min-1) using the known (/sup 14/C)palmitate infusion rate and the arterial plasma (/sup 14/C)-to-(/sup 3/H)palmitate ratio. In contrast, the mixed venous (/sup 14/C)-to-(/sup 3/H)palmitate ratio resulted in overestimates (P less than 0.05) of the actual (/sup 3/H)palmitate infusion rate. In summary, venous tracer infusion with arterial blood sampling for FFA tracer studies provides the most accurate estimates of tracee rate of appearance.

• Research Organization: Mayo Medical School, Rochester, MN (USA)
• OSTI ID: 7196075
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 254; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARTERIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD VESSELS
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DOGS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEINS
VERTEBRATES