Assessment of mineral dust cytotoxicity toward rat alveolar macrophages using a /sup 51/Cr release assay

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Fundam. Appl. Toxicol.; (United States)

DOI:https://doi.org/10.1016/0272-0590(88)90322-3· OSTI ID:7194767

Donaldson, K; Brown, G M

An assay was developed to assess the cytotoxicity of mineral dust by measuring release of /sup 51/Cr from prelabeled rat alveolar macrophages. Optimal conditions for the assay are described, the most notable being use of 2% albumin instead of fetal calf serum. The assay demonstrated loss of label into the supernatant when prelabeled macrophages were cultured with the two pathogenic mineral dusts, quartz and chrysotile asbestos. In contrast the inert mineral dust titanium dioxide had very little effect on /sup 51/Cr release by rat alveolar macrophages.

• Research Organization: Institute of Occupational Medicine, Edinburgh (England)
• OSTI ID: 7194767
• Journal Information: Fundam. Appl. Toxicol.; (United States), Journal Name: Fundam. Appl. Toxicol.; (United States) Vol. 10:2; ISSN FAATD
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ASBESTOS
BETA DECAY RADIOISOTOPES
CHALCOGENIDES
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DUSTS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MACROPHAGES
MAMMALS
MINERALS
NUCLEI
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
PHAGOCYTES
QUARTZ
RADIOASSAY
RADIOISOTOPES
RATS
RODENTS
SILICON COMPOUNDS
SILICON OXIDES
SOMATIC CELLS
TOXICITY
TRACER TECHNIQUES
VERTEBRATES