Protein synthesis inhibitors attenuate water flow in vasopressin-stimulated toad urinary bladder

Journal Article · Fri Jan 01 04:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:7191740

Hoch, B S; Ast, M B; Fusco, M J; Jacoby, M; Levine, S D ^[1]

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1. Albert Einstein College of Medicine, Bronx, NY (USA)

Vasopressin stimulates the introduction of aggregated particles, which may represent pathways for water flow, into the luminal membrane of toad urinary bladder. It is not known whether water transport pathways are degraded on removal from membrane or whether they are recycled. The authors examined the effect of the protein synthesis inhibitors cycloheximide and puromycin using repeated 30-min cycles of vasopressin followed by washout of vasopressin, all in the presence of an osmotic gradient, a protocol that maximizes aggregate turnover. High dose cycloheximide inhibited flow immediately. Low dose cycloheximide did not affect initial flow. In the absence of vasopressin, inhibition did not develop. Despite the inhibition of flow in vasopressin-treated tissues, the cAMP-dependent protein kinase ratio was elevated in cycloheximide-treated tissues, suggesting modulation at a distal site in the stimulatory cascade. ({sup 14}C)urea permeability was not inhibited by cycloheximide. Puromycin also inhibited water flow by the fourth challenge with vasopressin. The data suggest that protein synthesis inhibitors attenuate flow at a site that is distal to cAMP-dependent protein kinase. However, the reversal of inhibition in MIX-treated tissues suggests that the water pathway can be fully manifested given suitable stimulation. They conclude that either large stores of the transport system are available or that the transport system is extensively recycled on retrieval from the membrane.

• OSTI ID: 7191740
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 254:1; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMP
AMPHIBIANS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTINEOPLASTIC DRUGS
AQUATIC ORGANISMS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLADDER
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBONIC ACID DERIVATIVES
CHEMISTRY
CYCLOHEXIMIDE
DRUGS
ENZYMES
FUNGICIDES
HORMONES
LABELLED COMPOUNDS
MATERIALS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PERMEABILITY
PESTICIDES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PHYSIOLOGY
PITUITARY HORMONES
PUROMYCIN
TOADS
TRANSFERASES
UPTAKE
UREA
URINARY TRACT
URINE
VASOPRESSIN
VERTEBRATES
WASTES