Toward a physical map of the genome of the nematode Caenorhabditis elegans
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described. The technique is being applied to fragments from the nematode Caenorhabditis elegans (i) to facilitate cross-indexing of clones emanating from different laboratories and (ii) to construct a physical map of the genome. Eight hundred sixty clusters of clones, from 35 to 350 kilobases long and totaling about 60% of the genome, have been characterized.
- Research Organization:
- Medical Research Council Library of Molecular Biology, Cambridge (England)
- OSTI ID:
- 7191488
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ASCHELMINTHES
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
COMPUTER CODES
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ELECTROPHORESIS
EVEN-ODD NUCLEI
GENETIC MAPPING
GENOTYPE
HELMINTHS
ISOTOPES
LIGHT NUCLEI
MAPPING
NEMATODES
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ASCHELMINTHES
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
COMPUTER CODES
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ELECTROPHORESIS
EVEN-ODD NUCLEI
GENETIC MAPPING
GENOTYPE
HELMINTHS
ISOTOPES
LIGHT NUCLEI
MAPPING
NEMATODES
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES