Role of nitroreduction in the synergistic mutational response induced by mixtures of 1- and 3-nitrobenzo(a)pyrene in Salmonella typhimurium

Journal Article · Sat Dec 31 23:00:00 EST 1988 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850130303· OSTI ID:7190591

Thornton-Manning, J R; Campbell, W L; Hass, B S; Chen, J J; Fu, P P; Cerniglia, C E; Heflich, R H ^[1]

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1. National Center for Toxicological Research, Jefferson, AK (USA)

Previous studies showed that binary mixtures of the environmental pollutants 1- and 3-nitrobenzo(a)pyrene produced a synergistic mutational response in the Salmonella reversion assay. Since nitroreduction is believed to mediate the direct-acting mutagenicity of the individual compounds, the authors have examined the role of nitroreduction in the mutagenicity of mixtures of 1- and 3-nitrobenzo(a)pyrene in the Salmonella plate incorporation assay. While mixtures of 1- and 3-nitrobenzo(a)pyrene induced up to 183% more revertants in strain TA98 than produced by equivalent amounts of the individual compounds, in the nitroreductase-deficient strain TA98NR the same mixtures only induced up to 57% more revertants than the individual compounds. Analysis of mixtures of 1- and 3-nitrosobenzo(a)pyrene for mutation induction in TA98 yielded no evidence of a synergistic effect between the compounds. Salmonella cultures were also incubated with mixtures of 1- and 3-nitrobenzo(a)pyrene, as well as with equivalent amounts of the individual compounds. In two experiments, nitroreductive ability, as measured by the amount of 1-nitropyrene metabolized to 1-aminopyrene in 1 hr, was increased 9 to 105% in cultures pretreated with the mixtures as compared with cultures pretreated with the individual compounds. The results of this study support the hypothesis that nitroreduction is a major factor in the synergistic mutatonal response induced by 1- and 3-nitrobenzo(a)pyrene in Salmonella typhimurium.

• OSTI ID: 7190591
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 13:3; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BENZOPYRENE
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
CONDENSED AROMATICS
GENETIC EFFECTS
HYDROCARBONS
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENESIS
MUTANTS
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
REDUCTION
REVERTANTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SYNERGISM