Analysis of proteins associated with aluminum toxicity in wheat roots using 2-D page and computerized image processing

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:7189413

Ownby, J; Fischer, L ^[1]; Hruschka, W ^[2]

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1. Oklahoma State Univ., Stillwater (USA)
2. UDSA/ARS, Beltsville, MD (USA)

Experiments were conducted to determine if aluminum toxicity causes specific changes in the pattern of protein synthesis in wheat root tips, and if these changes are correlated with tolerance to Al in different cultivars. Proteins from cytoplasmic and microsomal fractions were separated by 2-D PAGE. Computerized image processing was used to enhance gel images, improve gel-to-gel registration, and quantify differences between composites of replicate gels. The primary response to Al toxicity was the enhanced formation of a subset of 8-10 cytoplasmic and 3-5 microsomal proteins, all of which were common to the 4 cultivars examined, including Al-sensitive Victory and Al-tolerant Atlas. Exposure to Al did not result in formation of any major proteins unique to tolerant cultivars. These results do not support the idea that inducible proteins mediate variable tolerance to Al in wheat cultivars.

• OSTI ID: 7189413
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALUMINIUM
BIOSYNTHESIS
CEREALS
ELECTROPHORESIS
ELEMENTS
GRASS
IMAGE PROCESSING
LILIOPSIDA
MAGNOLIOPHYTA
METALS
ORGANIC COMPOUNDS
PLANTS
PROCESSING
PROTEINS
ROOTS
SYNTHESIS
TOLERANCE
TOXICITY
WHEAT