Properties of a protein associated with metal toxicity in wheat

Conference · Wed May 01 04:00:00 EDT 1991 · Plant Physiology, Supplement; (United States)

OSTI ID:5598989

Cruz-Ortega, R; Owenby, J ^[1]

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1. Oklahoma State Univ., Stillwater (United States)

In previous studies it has been demonstrated that aluminum toxicity induces changes in the pattern of proteins synthesized in wheat. One of the most conspicuous changes is the appearance of a new cytoplasmic protein, with a pI of 5.2 and 18.6 KD MW. This polypeptide, tentatively called alumitin, has been observed in five wheat cultivars, of differing sensitivity to Al, at concentrations that inhibit root growth. In the present study, 2D-PAGE was used to present evidence that a polypeptide with the characteristics of alumitin is induced in wheat cv Victory after 24h treatment of growth-inhibiting levels of Cu (8{mu}M) and Cd (100{mu}M). However, it is not induced by low pH (3.5), Ca deficiency, or heat shock (4h, 37C) treatments sufficient to inhibit root growth. These results suggest that alumitin represents a specific response to metal toxicity rather than a general response to environmental stress. Rate of synthesis and turnover of alumitin are currently being studied.

• OSTI ID: 5598989
• Report Number(s): CONF-9107184--
• Conference Information: Journal Name: Plant Physiology, Supplement; (United States) Journal Volume: 96:1
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMICAL REACTION KINETICS
CADMIUM
CEREALS
COPPER
ELEMENTS
GRAMINEAE
GROWTH
KINETICS
LILIOPSIDA
MAGNOLIOPHYTA
METALS
ORGANIC COMPOUNDS
PEPTIDES
PLANT GROWTH
PLANTS
POLYPEPTIDES
PROTEINS
REACTION KINETICS
ROOTS
TOXICITY
TRANSITION ELEMENTS
WHEAT