Endothelium-derived relaxing factor (nitric oxide) has protective actions in the stomach

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(89)90540-7· OSTI ID:7189379

MacNaughton, W K; Wallace, J L ^[1]; Cirino, G ^[2]

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1. Univ. of Calgary, Alberta (Canada)
2. Univ. of Naples (Italy)

The role that nitric oxide, an endothelium-derived relaxing factor, may play in the regulation of gastric mucosal defense was investigated by assessing the potential protective actions of this factor against the damage caused by ethanol in an ex vivo chamber preparation of the rat stomach. Topical application of glyceryl trinitrate and sodium nitroprusside, which have been shown to release nitric oxide, markedly reduced the area of 70% ethanol-induced hemorrhagic damage. Topical application of a 0.01% solution of authentic nitric oxide also significantly reduced the severity of mucosal damage. Pretreatment with indomethacin precluded the involvement of endogenous prostaglandins in the protective effects of these agents. The protective effects of NO were transient, since a delay of 5 minutes between NO administration and ethanal administration resulted in a complete loss of the protective activity. The protection against ethanol afforded by 10 ug/ml nitroprusside could be completely reversed by intravenous infusion of either 1% methylene blue or 1 mM hemoglobin, both of which inhibit vasodilation induced by nitric oxide. Intravenous infusion of 1% methylene blue significantly increased the susceptibility of the mucosa to damage induced by topical 20% ethanol.

• OSTI ID: 7189379
• Journal Information: Life Sciences; (USA), Vol. 45:20; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ETHANOL
TOXICITY
NITRIC OXIDE
BIOLOGICAL EFFECTS
STOMACH
HEMORRHAGE
ENDOTHELIUM
METHYLENE BLUE
MUCOUS MEMBRANES
PROSTAGLANDINS
RATS
VASODILATION
ALCOHOLS
AMINES
ANIMAL TISSUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIMICROBIAL AGENTS
AZINES
BODY
CHALCOGENIDES
CHLORIDES
CHLORINE COMPOUNDS
DIGESTIVE SYSTEM
DRUGS
GASTROINTESTINAL TRACT
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MAMMALS
MEMBRANES
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PHENOTHIAZINES
RODENTS
SYMPTOMS
TISSUES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology