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Title: Mutations in a gene encoding the. cap alpha. subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling

Abstract

Mutations which allowed conjugation by Saccharomyces cerevisiae cells lacking a mating pheromone receptor gene were selected. One of the genes defined by such mutations was isolated from a yeast genomic library by complementation of a temperature-sensitive mutation and is identically to the gene GPA1 (also known as SCG1), recently shown to be highly homologous to gene encoding the ..cap alpha.. subunits of mammalian G proteins. Physiological analysis of temperature-sensitive gpal mutations suggests that the encoded G protein is involved in signaling in response to mating pheromones. Mutational disruption of G-protein activity causes cell-cycle arrest in G/sub 1/, deposition of mating-specific cell surface aggultinins, and induction of pheromone-specific mRNa, all of which are responses to pheromone in wild-type cells. In addition, mutants can conjugate without the benefit of mating pheromone or pheromone receptor. A model is presented where the activated G protein has a negative impact on a constitutive signal which normally keeps the pheromone response repressed.

Authors:
; ;
Publication Date:
Research Org.:
Dept. of Biology, College of Natural Sciences, Jeonbug National Univ., Jeonju, Jeonbug 520 (KR)
OSTI Identifier:
7188572
Resource Type:
Journal Article
Resource Relation:
Journal Name: Mol. Cell. Biol.; (United States); Journal Volume: 8:6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; GTP-ASES; BIOLOGICAL FUNCTIONS; GENE MUTATIONS; GENETIC MAPPING; PHEROMONE; BIOLOGICAL MODELS; BIOLOGICAL PATHWAYS; ENZYME ACTIVITY; MESSENGER-RNA; RECEPTORS; SACCHAROMYCES CEREVISIAE; TEMPERATURE EFFECTS; ACID ANHYDRASES; ENZYMES; FUNCTIONS; FUNGI; HYDROLASES; MAPPING; MEMBRANE PROTEINS; MICROORGANISMS; MUTATIONS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PLANTS; PROTEINS; RNA; SACCHAROMYCES; SECRETION; YEASTS; 550200* - Biochemistry; 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)

Citation Formats

Jahng, K.Y., Ferguson, J., and Reed, S.I. Mutations in a gene encoding the. cap alpha. subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling. United States: N. p., 1988. Web. doi:10.1128/MCB.8.6.2484.
Jahng, K.Y., Ferguson, J., & Reed, S.I. Mutations in a gene encoding the. cap alpha. subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling. United States. doi:10.1128/MCB.8.6.2484.
Jahng, K.Y., Ferguson, J., and Reed, S.I. Wed . "Mutations in a gene encoding the. cap alpha. subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling". United States. doi:10.1128/MCB.8.6.2484.
@article{osti_7188572,
title = {Mutations in a gene encoding the. cap alpha. subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling},
author = {Jahng, K.Y. and Ferguson, J. and Reed, S.I.},
abstractNote = {Mutations which allowed conjugation by Saccharomyces cerevisiae cells lacking a mating pheromone receptor gene were selected. One of the genes defined by such mutations was isolated from a yeast genomic library by complementation of a temperature-sensitive mutation and is identically to the gene GPA1 (also known as SCG1), recently shown to be highly homologous to gene encoding the ..cap alpha.. subunits of mammalian G proteins. Physiological analysis of temperature-sensitive gpal mutations suggests that the encoded G protein is involved in signaling in response to mating pheromones. Mutational disruption of G-protein activity causes cell-cycle arrest in G/sub 1/, deposition of mating-specific cell surface aggultinins, and induction of pheromone-specific mRNa, all of which are responses to pheromone in wild-type cells. In addition, mutants can conjugate without the benefit of mating pheromone or pheromone receptor. A model is presented where the activated G protein has a negative impact on a constitutive signal which normally keeps the pheromone response repressed.},
doi = {10.1128/MCB.8.6.2484},
journal = {Mol. Cell. Biol.; (United States)},
number = ,
volume = 8:6,
place = {United States},
year = {Wed Jun 01 00:00:00 EDT 1988},
month = {Wed Jun 01 00:00:00 EDT 1988}
}