RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus
The yeast rna mutations (rna2 through rna10/11) are a set of temperature-sensitive mutations that result in the accumulation of pre-mRNAs at the nonpermissive temperature. Most of the yeast RNA gene products are involved in and essential for mRNA splicing in vitro, suggesting that they code for components of the splicing machinery. We tested this proposal by using an in vitro-synthesized RNA11 protein to complement the temperature-sensitive defect of the rna11 extract. During the in vitro complementation, the input RNA11 protein was associated with the 40S spliceosome and a 30S complex, suggesting that the RNA11 protein is indeed a component of the spliceosome. The formation of the RNA11-associated 30S complex did not require any exogenous RNA substrate, suggesting that this 30S particle is likely to be a preassembled complex involved in splicing. The RNA11-specific antibody inhibited the mRNA splicing in vitro, confirming the essential role of the RNA11 protein in mRNA splicing. Finally, using the anti-RNA11 antibody, they localized the RNA11 protein to the periphery of the yeast nucleus.
- Research Organization:
- Div. of Biology, California Institute of Technology, Pasadena, CA (US)
- OSTI ID:
- 7188563
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:6; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL FUNCTIONS
CLEAVAGE
CRYSTAL STRUCTURE
FUNCTIONS
FUNGI
GENE MUTATIONS
IMMUNOASSAY
IN VITRO
MESSENGER-RNA
MICROORGANISMS
MICROSTRUCTURE
MOLECULAR BIOLOGY
MUTATIONS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
RNA
RNA PROCESSING
STRUCTURE-ACTIVITY RELATIONSHIPS
SUBSTRATES
TEMPERATURE EFFECTS
YEASTS