A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: A potential homologue of the yeast PRP8 protein
- Massachusetts Institute of Technology, Cambridge (USA)
A mammalian protein of approximately 220 kDa (p220) was UV-crosslinked to precursor mRNAs (pre-mRNAs) under splicing conditions. The kinetics and biochemical requirements of the UV-crosslinking of p220 corresponded to the kinetics and biochemical requirements of spliceosome formation. On Western blots, antibodies against the yeast splicing factor PRP8 recognized a doublet of proteins, the faster migrating of which comigrated with p220. Furthermore, UV-crosslinked p220 was immunoprecipitated with anti-PRP8 antisera. These results suggest structural conservation of the splicing factor PRP8 from yeast to mammals and show that this protein is in close proximity to the pre-mRNA in the spliceosome.
- OSTI ID:
- 6833753
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:8; ISSN PNASA; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3{prime} splice site selection
RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus
Related Subjects
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CELL NUCLEI
CHEMICAL REACTIONS
CROSS-LINKING
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EUMYCOTA
FUNGI
HELA CELLS
IMMUNOASSAY
KINETICS
MAMMALS
MAN
MESSENGER-RNA
MICROORGANISMS
MOLECULAR WEIGHT
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PLANTS
POLYMERIZATION
PRECURSOR
PRIMATES
PROTEINS
RADIATIONS
REACTION KINETICS
RNA
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
ULTRAVIOLET RADIATION
VERTEBRATES
YEASTS