Regulation of intracellular calcium in resting and stimulated rat basophilic leukemia cells

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:7188120

Mohr, F C

Intracellular calcium regulation was studied in a cell line of mast cells, the rat basophilic leukemia (RBL) cells with the purpose of determining (1) The properties of the plasma membrane calcium permeability pathway and (2) The role of intracellular calcium stores. The first set of experiments showed that depolarization did not induce calcium entry or secretion in resting cells and did inhibit antigen-stimulated calcium uptake and secretion. In the second set of experiments the ionic basis of antigen-induced depolarization was studied using the fluorescent potential-sensitive probe bis-oxonol. The properties of the calcium entry pathway were more consistent with a calcium channel than a calcium transport mechanism such as Na:Ca exchange. The third set of experiments examined the effects of the proton ionophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) on RBL cells. CCCP inhibited antigen-stimulated /sup 45/Ca uptake and secretion by depolarizing the plasma membrane.

• Research Organization: Cornell Univ., Ithaca, NY (USA)
• OSTI ID: 7188120
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM COMPOUNDS
MEMBRANE TRANSPORT
SECRETION
BIOLOGICAL PATHWAYS
CALCIUM 45
CELL MEMBRANES
LEUKEMIA
RATS
TRACER TECHNIQUES
TUMOR CELLS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANES
NEOPLASMS
NUCLEI
RADIOISOTOPES
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques