Protein phosphorylation in rat basophilic leukemia cells following stimulation for histamines release

Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5495627

Essani, N; Essani, K; Siraganian, R P

The stimulation of the rat basophilic leukemia 2H3 (RBL-2H3) cells results in protein phosphorylation which accompanies the secretion of histamine. In the present investigations the cells were stimulated either through the IgE receptor or with the calcium ionophore A23187. Total cellular proteins were analyzed by 2-dimensional electrophoresis. The pattern of changes in /sup 32/P incorporation into polypeptides was similar following both antigen- or calcium ionophore-stimulation. At least 14 polypeptides demonstrated increased /sup 32/P incorporation following activation with either antigen or ionophore. Twelve additional phosphopolypeptides were detected in the stimulated cells which could not be seen in the absence of activation. There was one polypeptide with dephosphorylation following stimulation. Time course experiments revealed that the overall pattern of phosphorylation with both secretagogues was similar. Only 2 polypeptides were found not to be phosphorylated in the absence of extracellular Ca/sup 2 +/. The results indicate the phosphorylation of a complex number of polypeptides during the secretory event. It also demonstrated that the phosphorylation of most of these occur in the absence of extracellular Ca/sup 2 +/ and are events which precede the rise in cytoplasmic Ca/sup 2 +/ concentration.

• Research Organization: N.I.H., Bethesda, MD
• OSTI ID: 5495627
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
AMINES
ANIMAL CELLS
ANIMALS
ANTIGENS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM COMPOUNDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTROPHORESIS
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
ISOTOPES
LEUKEMIA
LIGHT NUCLEI
MAMMALS
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SECRETION
TUMOR CELLS
VERTEBRATES