Maturation of Xenopus oocytes. I. Facilitation by ouabain

Journal Article · Wed Dec 31 23:00:00 EST 1975 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(76)90654-6· OSTI ID:7187546

Vitto, A Jr ^[1]; Wallace, R A

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1. Univ. of Tennessee, Oak Ridge,

Ouabain, at a concentration which effectively suppresses Rb/sup +/ uptake into isolated, full-grown oocytes facilitates progesterone-stimulated maturation; germinal vesicle breakdown occurs several hours sooner in maximally stimulated oocytes or occurs with increased frequency in marginally stimulated oocytes. A similar facilitation is observed with strophanthidin and in K/sup +/-deficient media. Ouabain acts directly on the oocyte rather than the investing layer of follicle cells. Oocytes, removed from their follicles, begin to accumulate K/sup +/ and lose Na/sup +/, a process which is blocked by ouabain. This process, or the altered intracellular environment which results, is apparently antagonistic to progesterone-stimulated oocyte maturation.

• OSTI ID: 7187546
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 97; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ALKALI METALS
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARDIOTONICS
DRUGS
ELEMENTS
GERM CELLS
GLYCOSIDES
GROWTH
HORMONES
KETONES
METALS
OOCYTES
ORGANIC COMPOUNDS
OUABAIN
POTASSIUM
PREGNANES
PROGESTERONE
RUBIDIUM
SODIUM
STEROID HORMONES
STEROIDS
STROPHANTIN
VERTEBRATES