Effects of copper and cadmium on electrophoretically detected enzymes in fish

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1987

OSTI ID:7183680

Chagnon, N L

The in vitro effects of copper and cadmium on the activity of electrophoretically detected enzymes in 16 fish species from 7 locations were determined by adding the metals to the biochemical stains used to resolve the enzyme systems on a starch gel. This assay technique was used successfully to demonstrate the direct effects of copper on the activity of phosphoglucomutase-2 (PGM-2), glucose phosphate isomerase (GPI-2), and isocitrate dehydrogenase-2 (ICD-2) in mosquitofish (Gambusia affinis) and to show that differences exist in the copper sensitivity of intraspecific PGM-2 allozymes and interspecific PGM-2 enzymes in a number of fish species tested. It was possible to detect copper sensitive and copper tolerant isoalleles at the PGM-2 locus in mosquitofish. Cadmium did not have any in vitro effects on the activity of the enzyme systems tested.

• Research Organization: Miami Univ., FL (USA)
• OSTI ID: 7183680
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOLOGICAL VARIABILITY
CADMIUM
COPPER
ELECTROPHORESIS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
FISHES
GENETIC VARIABILITY
IN VITRO
ISOMERASES
METABOLISM
METALS
OXIDOREDUCTASES
SENSITIVITY
TRANSITION ELEMENTS
VERTEBRATES