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Acute effects of heavy metals and low pH on the genetic structure of Hyalella azteca

Conference ·
OSTI ID:218424
; ; ; ;  [1]
  1. Miami Univ., Oxford, OH (United States). Dept. of Zoology
Groups of approximately 500 adult Hyalella azteca were exposed to concentrations of Cd, Cu, Pb, Zn or low pH for 10 days resulting in 80% mortality. Allozyme analysis of the survivors was then performed using 7 polymorphic loci: acid phosphatase (ACP), adenosine deaminase (ADA), aldehyde dehydrogenase (ALDH), fumarate hydratase (FUM), glucose-6-phosphate isomerase (GPI), mannose phosphate isomerase (MPI) and phosphoglucomutase (PGM). The genotype frequencies of the survivors differed significantly from the control at all loci except for ACP in the copper and low pH exposures and GPI in the Cd exposure. The distribution of alleles at 7 loci was significantly different among groups. No differences in heterozygosity among groups were observed. Examination of both genotype and allele proportions indicated an increase in PGM-bb and PGM-B in all exposures. The possible use of Hyalella allozyme analysis as a bioindicator will be discussed in terms of enzyme activity, variability and sensitivity.
OSTI ID:
218424
Report Number(s):
CONF-9511137--; ISBN 1-880611-03-1
Country of Publication:
United States
Language:
English

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