Effects of controlled RAD52 expression on repair and recombination in Saccharomyces cerevisiae

Journal Article · Mon Apr 01 04:00:00 EST 1991 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.11.4.2013· OSTI ID:7175876

Dornfeld, K J; Livingston, D M ^[1]

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1. University of Minnesota, Minneapolis (United States)

The authors have examined the effects of RAD52 overexpression on methyl methanesulfonate (MMS) sensitivity and spontaneous mitotic recombination rates. Cells expressing a 10-fold excess of RAD52 mRNA from the ENO1 promoter are no more resistant to MMS than are wild-type cells. Similarly, under the same condition, the rate of mitotic recombination within a reporter plasmid does not exceed that measured in wild-type cells. This high level of expression is capable of correcting the defects of rad52 mutant cells in carrying out repair and recombination. From these observations, they conclude that wild-type amounts of Rad52 are not rate limiting for repair of MMS-inducted lesions or plasmid recombination. By placing RAD52 under the control of the inducible GAL1 promoter, they find that induction results in a 12-fold increase in the fraction of recombinants within 4 h. After this time, the fraction increases less rapidly. When RAD52 expression is quickly repressed during induction, the amount of RAD52 mRNA decreases rapidly and no nascent recombinants are formed. This result suggests a short active half-life for the protein product. Induction of RAD52 in G1-arrested mutant cells also causes a rapid increase in recombinants, suggesting that replication is not necessary for plasmid recombination.

• OSTI ID: 7175876
• Journal Information: Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 11:4; ISSN 0270-7306; ISSN MCEBD4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL CYCLE
CELL DIVISION
ESTERS
EUMYCOTA
FUNGI
GENE RECOMBINATION
GENE REGULATION
METHYL METHANESULFONATE
MICROORGANISMS
MITOSIS
MUTAGENS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
PLASMIDS
RADIOSENSITIVITY
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SENSITIVITY
SULFONIC ACID ESTERS
YEASTS