Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Regulation of sphingolipid synthesis in renal cells from normal subjects and familial hypercholesterolemic subjects

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7171353
; ;

The authors have investigated the effects of lipoproteins on sphingolipid metabolism in proximal renal tubular cells from normal subjects and low density lipoprotein (LDL) receptor-negative homozygous familial hypercholesterolemic (FH) subjects (TB,BA,DD,VH) employing (/sup 3/H)serine and (/sup 3/H)glucose. The results were: normal cells - 1) LDL (25 ..mu..g/ml) decreased the incorporation of (/sup 3/H)glucose and (/sup 3/H)serine into ceramide and LacCer about 2-3 fold; 2) the incorporation of (/sup 3/H)serine into sphingomyelin was also reduced 2 fold by LDL; 3) LDL modified by reductive methylation of lysine residues (which is not recognized by the LDL receptor) did not decrease the incorporation of (/sup 3/H)glucose and (/sup 3/H)serine into sphingolipids; FH cells - In contrast to normal cells, LDL (100 ..mu..g/ml) stimulated both the incorporation of (/sup 3/H)glucose into LacCer and of (/sup 3/H)serine into ceramide, LacCer and sphingomyelin 2-3 fold in cells lacking LDL receptors. The authors conclude that the endogenous synthesis of sphingolipids in normal renal cells may be regulated by the LDL receptor. Lack of LDL receptor, as in FH cells, results in increased sphingolipid synthesis and storage of LacCer.

Research Organization:
Johns Hopkins Univ., Baltimore, MD
OSTI ID:
7171353
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

Similar Records

Differences in ( sup 14 C)glycerol utilization in normal and familial hypercholesterolemic fibroblasts
Journal Article · Mon Dec 31 23:00:00 EST 1990 · Artery (Fulton, Michigan); (USA) · OSTI ID:5642819
Sphingolipid synthesis deficiency in a mutant of Bacteroides levii
Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6986122
Substrate availability for long-chain base formation as a regulator of hepatic sphingolipid and cholesterol biosynthesis
Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6965996

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINO ACIDS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHOLESTEROL
ESTERS
GLUCOSE
HEXOSES
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KIDNEYS
LABELLED COMPOUNDS
LIPIDS
LIPOPROTEINS
MEMBRANE PROTEINS
METABOLISM
METHYLATION
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
PROTEINS
RECEPTORS
SACCHARIDES
SERINE
SPHINGOMYELINS
STEROIDS
STEROLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS