Differences in ( sup 14 C)glycerol utilization in normal and familial hypercholesterolemic fibroblasts

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Artery (Fulton, Michigan); (USA)

OSTI ID:5642819

Shireman, R B; Durieux, J ^[1]

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1. Univ. of Florida Gainesville (USA)

It is known that cultured fibroblasts from familial hypercholesterolemia (FH) patients lack the normal cell receptor for low density lipoprotein (LDL) and that the absence of receptor-mediated transport of LDL cholesterol into these cells results in increased cellular synthesis of cholesterol. After 20 h perincubation in lipid-free medium, cultured FH fibroblasts incorporated significantly greater amounts of ({sup 14}C)glycerol into cellular lipids than did normal fibroblasts. Relative to the control medium which contained only bovine serum albumin (BSA), preincubation with 5% fetal bovine serum or 50 micrograms LDL/ml decreased ({sup 14}C)glycerol incorporation by both cell types. FH cells utilized more ({sup 14}C)glycerol for phospholipid synthesis and less for triglyceride synthesis than normal cells. This study indicates that LDL may be important in the transport of glycerides, as well as cholesterol, to cells.

• OSTI ID: 5642819
• Journal Information: Artery (Fulton, Michigan); (USA), Journal Name: Artery (Fulton, Michigan); (USA) Vol. 18:2; ISSN 0098-6127; ISSN ARTED
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMAL CELLS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CHOLESTEROL
CONNECTIVE TISSUE CELLS
DISEASES
ESTERS
FIBROBLASTS
GLYCEROL
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
METABOLIC DISEASES
METABOLISM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PATHOGENESIS
PHOSPHOLIPIDS
SOMATIC CELLS
STEROIDS
STEROLS
SYNTHESIS
TRACER TECHNIQUES