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Inhibition of HDL binding by tetranitromethane is not related to cross-linking of phospholipids to apoproteins

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7171104
; ; ;
The authors have shown that treatment of human HDL with tetranitromethane (TNM) inhibits the lipoprotein specific binding to rat liver plasma membranes and to cultured rat hepatoma cells. In addition to the expected nitration of tyrosine residues, cross-linking of lipids to apoproteins and apoproteins to one another occurred during treatment of HDL with TNM. In order to determine the role of cross-linking of phospholipids to apoproteins they prepared a reconstituted HDL in which the native phospholipids were replaced with dimyristoylphosphatidylcholine (DMPC), which has no double bonds in the acyl chains. The reconstituted HDL (DMPC-HDL) has properties, including the ability to bind to HDL binding sites, similar to those of native HDL. On nitration with TNM, DMPC-HDL, like native HDL, lost its ability to bind to the HDL binding sites of isolated rat liver and rat testes plasma membranes as determined by competitive binding with /sup 125/I-HDL. Nitrated DMPC-HDL contained only traces of phospholipids covalently linked to apoproteins, whereas 21% of phospholipids were linked to apoproteins of nitrated native HDL; cross-linking of apoproteins to themselves was detected in both nitrated HDL's. These results show that covalent cross-linking of phospholipids to apoproteins is not responsible for the inhibition of HDL binding by TNM.
Research Organization:
Medical College of Pennsylvania, Philadelphia
OSTI ID:
7171104
Report Number(s):
CONF-8606151-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CHEMICAL REACTIONS
COVALENCE
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
GLANDS
GONADS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPIDS
LIPOPROTEINS
LIVER
MALE GENITALS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
TESTES
TRACER TECHNIQUES
VERTEBRATES