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Tetranitromethane modification of HDL: cross-linking as a possible mechanism for the inhibition of specific HDL binding to membranes

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6065455
; ;
Treatment of HDL with tetranitromethane (TNM) inhibits the lipoprotein's specific binding to cells and isolated membranes. TNM causes the nitration of tyrosine residues, and the cross-linking of apoproteins to each other and to lipids in HDL particles. They recently showed that cross-linking of apoproteins to phospholipids was not responsible for the inhibition of binding. To determine the role of cross-linking of apoproteins to each other in the inhibition, they used the bifunctional reagent dimethyl suberimidate (DMS) to cross-link the apoproteins in human HDL/sub 3/. Over 80% of apoproteins in DMS-HDL/sub 3/ were cross-linked, as analyzed by SDS-PAGE. However, DMS-HDL/sub 3/ was similar to control HDL/sub 3/ in its lipid composition and size distribution. As determined by competitive binding with /sup 125/I-HDL/sub 3/, DMS-HDL/sub 3/ was almost completely unable to bind specifically to rat liver plasma membranes. These results suggest that cross-linking of apoproteins to each other is the mechanism by which TNM treatment inhibits the specific binding of HDL to membranes.
Research Organization:
Medical College of Pennsylvania, Philadelphia
OSTI ID:
6065455
Report Number(s):
CONF-870644-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDITIVES
ANIMALS
APOLIPOPROTEINS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
FUEL ADDITIVES
FUNCTIONS
GLANDS
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPIDS
LIPOPROTEINS
LIVER
MAMMALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
REAGENTS
RODENTS
TRACER TECHNIQUES
VERTEBRATES