Actions of mammalian insulin on a Neurospora variant: effects on intracellular metabolite levels as monitored by P-31 NMR spectroscopy
Fourier transform P-31 NMR spectroscopy (81 MHz) was used to investigate the biochemical nature of insulin action upon the cell wall-deficient slime mutant of Neurospora crassa. Spectra of oxygenated, living cells (ca.10/sup 9//ml.) in late logarithmic-early stationary phase of growth were accumulated for approximately 20 min. (350-450 pulses). Pronounced differences were seen in the metabolite levels of cells cultured for 18-21 hours in the presence of insulin (100 nM) as compared to cells cultured in its absence. Differences in the insulin-grown cells included higher levels of sugar phosphates, inorganic (cytoplasmic) phosphate, NAD+/NADH and UDP-glucose (UDPG) compared to control cells, in which UDP-N-acetylglucosamine (UDPNAG) was the prominent sugar nucleotide. When 100 mM glucose was administered with insulin immediately prior to measurement, short term effects were seen. There were significant increases of sugar phosphates, inorganic phosphate, NAD+/NADH, phosphodiesters and UDPG relative to the case of glucose addition alone. These results are wholly consistent with the known influence of insulin upon mammalian metabolism: stimulation of glucose uptake, phosphorylation and oxidation, phosphatide synthesis and Pi uptake.
- Research Organization:
- UMDNJ-Rutgers Medical School, Piscataway
- OSTI ID:
- 7170988
- Report Number(s):
- CONF-8606151-; TRN: 86-039117
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Actions of mammalian insulin on a Neurospora variant: morphology, growth and binding
In vivo NMR analysis of the influence of pyruvate decarboxylase and alcohol dehydrogenase of Zymomonas mobilis on the anaerobic metabolism of Escherichia coli
Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
INSULIN
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
NEUROSPORA
GROWTH
CELL CULTURES
GLUCOSAMINE
GLUCOSE
METABOLISM
METABOLITES
MUTANTS
NAD
NADH2
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
PHOSPHATES
PHOSPHORUS 31
ALDEHYDES
AMINES
CARBOHYDRATES
COENZYMES
FUNGI
HEXOSAMINES
HEXOSES
HORMONES
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MONOSACCHARIDES
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PLANTS
REACTION KINETICS
RESONANCE
SACCHARIDES
SPECTRA
STABLE ISOTOPES
550201* - Biochemistry- Tracer Techniques
550600 - Medicine