Actions of mammalian insulin on a Neurospora variant: effects on intracellular metabolite levels as monitored by P-31 NMR spectroscopy

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7170988

Greenfield, N J; McKenzie, M A; Jordan, F; Takahashi, M; Lenard, J

Fourier transform P-31 NMR spectroscopy (81 MHz) was used to investigate the biochemical nature of insulin action upon the cell wall-deficient slime mutant of Neurospora crassa. Spectra of oxygenated, living cells (ca.10/sup 9//ml.) in late logarithmic-early stationary phase of growth were accumulated for approximately 20 min. (350-450 pulses). Pronounced differences were seen in the metabolite levels of cells cultured for 18-21 hours in the presence of insulin (100 nM) as compared to cells cultured in its absence. Differences in the insulin-grown cells included higher levels of sugar phosphates, inorganic (cytoplasmic) phosphate, NAD+/NADH and UDP-glucose (UDPG) compared to control cells, in which UDP-N-acetylglucosamine (UDPNAG) was the prominent sugar nucleotide. When 100 mM glucose was administered with insulin immediately prior to measurement, short term effects were seen. There were significant increases of sugar phosphates, inorganic phosphate, NAD+/NADH, phosphodiesters and UDPG relative to the case of glucose addition alone. These results are wholly consistent with the known influence of insulin upon mammalian metabolism: stimulation of glucose uptake, phosphorylation and oxidation, phosphatide synthesis and Pi uptake.

• Research Organization: UMDNJ-Rutgers Medical School, Piscataway
• OSTI ID: 7170988
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550600 -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
AMINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CELL CULTURES
COENZYMES
FUNGI
GLUCOSAMINE
GLUCOSE
GROWTH
HEXOSAMINES
HEXOSES
HORMONES
INSULIN
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
METABOLISM
METABOLITES
MONOSACCHARIDES
MUTANTS
NAD
NADH2
NEUROSPORA
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PHOSPHATES
PHOSPHORUS 31
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PLANTS
REACTION KINETICS
RESONANCE
SACCHARIDES
SPECTRA
STABLE ISOTOPES