Structure and organization of a mouse proline-rich protein gene
Thesis/Dissertation
·
OSTI ID:7170727
One of the family of mouse proline-rich protein geners was isolated on a cloned 3.6 kilobase pair EcoR1/Bg1II-generated DNA fragment form a (partial) Sau3A bacteriophage library of Cd-1 mouse chromosomal DNA. Phage harboring the gene were identified by plaque hybridization using /sup 32/P-labeled cDNA inserts from clones pRP33 and pMP1, obtained from rat and mouse, respectively. The transcriptional unit includes three exonic sequences separated by 1434 base pairs (Intron I) and 450 base pairs (Intron II). The complete primary structure of the gene and the 5' and 3' flanking regions were determined by the Maxam and Gilbert sequencing method. Analysis of the nucleotide sequence 5' to the first exon indicates several sequences that might be involved in the induction and expression of a mouse proline-rich protein. The derived amino acid sequences revealed that this protein contains thirteen tandemly repeat regions of fourteen amino acids in length with prototype sequence PPPPGGPQPRPPQG. Each amino acid within the repeat has its favorite codon, and the consensus DNA sequence for each repeat is CCACCACCACCAGGAGGCCCACAGCCGAGACCCCCTCAAGGC. The high conservation in both nucleotide and amino acid sequences in the repeat region suggests that this gene may have evolved by gene duplication of a 42 base pair internal repeat and by gene conversion.
- Research Organization:
- Purdue Univ., Lafayette, IN (USA)
- OSTI ID:
- 7170727
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CARBOXYLIC ACIDS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
GENES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICE
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RADIOISOTOPES
RECOMBINANT DNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CARBOXYLIC ACIDS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
GENES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICE
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RADIOISOTOPES
RECOMBINANT DNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
VERTEBRATES