Nucleotide sequence of the gene for the b subunit of human factor XIII

Journal Article · Sat Dec 01 04:00:00 EST 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00503a007· OSTI ID:5527590

Bottenus, R E; Ichinose, A; Davie, E W ^[1]

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1. Univ. of Washington, Seattle (USA)

Factor XIII (M{sub r} 320 000) is a blood coagulation factor that stabilizes and strengthens the fibrin clot. It circulates in blood as a tetramer composed of two a subunits (M{sub r} 75 000 each) and two b subunits (M{sub r} 80 000 each). The b subunit consists of 641 amino acids and includes 10 tandem repeats of 60 amino acids known as GP-I structures, short consensus repeats (SCR), or sushi domains. In the present study, the human gene for the b subunit has been isolated from three different genomic libraries prepared in {lambda} phage. Fifteen independent phage with inserts coding for the entire gene were isolated and characterized by restriction mapping, Southern blotting, and DNA sequencing. The gene was found to be 28 kilobases in length and consisted of 12 exons (I-XII) separated by 11 intervening sequences. The leader sequence was encoded by exon I, while the carbonyl-terminal region of the protein was encoded by exon XII. Exons II-XI each coded for a single sushi domain, suggesting that the gene evolved through exon shuffling and duplication. The 12 exons in the gene ranged in size from 64 to 222 base pairs, while the introns ranged in size from 87 to 9970 nucleotides and made up 92{percent} of the gene. One nucleotide change was found in the coding region of the gene when its sequence was compared to that of the cDNA. This difference, however, did not result in a change in the amino acid sequence of the protein.

• OSTI ID: 5527590
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:51; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMALS
BLOOD COAGULATION FACTORS
COAGULANTS
CONNECTIVE TISSUE CELLS
DNA
DNA SEQUENCING
DRUGS
FIBRIN
FIBROBLASTS
GENES
HEMATOLOGIC AGENTS
HEMOSTATICS
MAMMALS
MAN
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RECOMBINANT DNA
SCLEROPROTEINS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES