Effect of an ozone injury-retardant chemical on isozyme profiles from alfalfa callus in vitro

Technical Report · Sat Jan 01 00:00:00 EST 1983

OSTI ID:7169868

Rier, J P; Sood, V K; Whitaker, A; Watson, C

Plant ozone injury retardant (EDU or ethylenediurea) at 1.0 ppm inhibited growth of callus of alfalfa cultivars Williamsburg (ozone-sensitive) and MSB-CW5An2(ozone-insensitive) germplasm of Medicago sative. The presence of EDU(0.1 ppm) in growth medium increased the number of protein and peroxidase isozyme bands in alfalfa cultivar stem callus and ozone modified their intensities. Protein profiles of MSB stem callus from media containing EDU or exposed to ozone were unchanged. Marked differences were observed between the peroxidase profiles of ozonated and control ozone-insensitive stem callus from media containing EDU. Protein profiles of ozonated ozone-insensitive leaf callus differed slightly from controls.

• Research Organization: Howard Univ., Washington, DC (USA). Dept. of Botany
• OSTI ID: 7169868
• Report Number(s): PB-86-229176/XAB
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
OZONE
BIOLOGICAL EFFECTS
PLANT GROWTH
ALFALFA
INHIBITION
ORGANIC COMPOUNDS
PEROXIDASES
PROTEINS
SENSITIVITY
BACTERIA
ENZYMES
GROWTH
LEGUMINOSAE
MICROORGANISMS
OXIDOREDUCTASES
PLANTS
RHIZOBIUM
560300* - Chemicals Metabolism & Toxicology