Effect of an ozone injury retardant chemical on isozyme profiles from alfalfa callus in vitro

Journal Article · Sat Jan 01 04:00:00 EST 1983 · Phytopathology; (United States)

DOI:https://doi.org/10.1094/Phyto-73-1262· OSTI ID:6884490

Rier, Jr, J P; Sood, V K; Whitaker, A; Watson, C

Plant ozone injury retardant N-(2-(2-oxo-1-imidazolidinyl)-ethyl)-N'-phenylurea (EDU or ethylenediurea) at 1.0 ppm inhibited growth of callus of alfalfa cultivars Williamsburg (ozone-sensitive) and MSB-CW5An2 (ozone-insensitive) germplasm of Medicago sativa. The presence of EDU (0.1 ppm)in the growth medium increased the number of protein and peroxidase isozyme bands in alfalfa cultivar Williamsburg stem callus and ozone modified their intensities. Protein profiles of MSB stem callus from media containing EDU or exposed to ozone were unchanged. Marked differences were observed between the peroxidase profiles of ozonated and control ozone-insensitive stem callus from media containing EDU. Protein profiles of ozonated ozone-sensitive leaf callus differed slightly from controls. The peroxidase profile of ozonated ozone-sensitive leaf callus was not altered when its growth medium contained EDU, but when it was absent, changes were observed in these profiles.

• Research Organization: Howard Univ., Washington, DC
• OSTI ID: 6884490
• Journal Information: Phytopathology; (United States), Journal Name: Phytopathology; (United States) Vol. 73:9; ISSN PHYTA
• Country of Publication: United States
• Language: English

Related Subjects

560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALFALFA
BIOCHEMISTRY
CHEMISTRY
CORRELATIONS
GROWTH
INHIBITION
INJURIES
LEGUMINOSAE
OZONE
PLANTS
VARIATIONS