Effect of an ozone injury-retardant chemical on isozyme profiles from alfalfa callus in vitro

Technical Report · Sat Jan 01 04:00:00 EST 1983

OSTI ID:7169868

Rier, J P; Sood, V K; Whitaker, A; Watson, C

Plant ozone injury retardant (EDU or ethylenediurea) at 1.0 ppm inhibited growth of callus of alfalfa cultivars Williamsburg (ozone-sensitive) and MSB-CW5An2(ozone-insensitive) germplasm of Medicago sative. The presence of EDU(0.1 ppm) in growth medium increased the number of protein and peroxidase isozyme bands in alfalfa cultivar stem callus and ozone modified their intensities. Protein profiles of MSB stem callus from media containing EDU or exposed to ozone were unchanged. Marked differences were observed between the peroxidase profiles of ozonated and control ozone-insensitive stem callus from media containing EDU. Protein profiles of ozonated ozone-insensitive leaf callus differed slightly from controls.

• Research Organization: Howard Univ., Washington, DC (USA). Dept. of Botany
• OSTI ID: 7169868
• Report Number(s): PB-86-229176/XAB
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALFALFA
BACTERIA
BIOLOGICAL EFFECTS
ENZYMES
GROWTH
INHIBITION
LEGUMINOSAE
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OZONE
PEROXIDASES
PLANT GROWTH
PLANTS
PROTEINS
RHIZOBIUM
SENSITIVITY