A compartmental model to quantitate in vivo glucose transport in the human forearm

Journal Article · Fri Dec 01 04:00:00 EST 1989 · American Journal of Physiology; (USA)

OSTI ID:7167738

Cobelli, C; Saccomani, M P; Ferrannini, E; Defronzo, R A; Gelfand, R; Bonadonna, R ^[1]

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1. Univ. of Padua (Italy)

Glucose transport is a critical step in the control of glucose disposal that, until presently, has not been quantitated in vivo in humans. We have employed the perfused forearm and euglycemic insulin-clamp techniques in combination with a dual-tracer injection to measure basal and insulin-mediated glucose transport in six normal subjects. L-({sup 3}H)glucose, which is not transported, was used to trace extracellular glucose kinetics; 3-O-({sup 14}C)-methyl-D-glucose, transportable but not metabolizable, was used to monitor glucose movement across the cell membrane. After bolus intra-arterial injection of the two tracers, plasma samples were obtained every 15-30 s for 10 min from a deep forearm vein to determine the washout curves. A linear compartmental model was developed that accounts for blood flow heterogeneity. It consists of three parallel, two-compartment chains merging into the sampling compartment to which cellular compartments are appended. A priori identifiability analysis was performed. The uniquely identifiable parameterization includes the transport rate constants of glucose into and out of the cell. The model was identified using nonlinear least-squares parameter estimation. Transport parameters are estimated with very good precision, and their reproducibility is satisfactory. The model also allows the estimation of the mean arteriovenous transit times of both the extracellular and the transported tracer. The compartmental model provides a novel approach to investigate glucose transport in vivo in humans.

• OSTI ID: 7167738
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 257; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
GLUCOSE
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
INSULIN
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
LEAST SQUARE FIT
MAMMALS
MAN
MATHEMATICAL MODELS
MAXIMUM-LIKELIHOOD FIT
MEMBRANE TRANSPORT
MEMBRANES
MONOSACCHARIDES
MUSCLES
NUMERICAL SOLUTION
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PRIMATES
SACCHARIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES