Toxicity of penicillic acid for rat alveolar macrophages in vitro. [Aspergillus; Penicillium]
Penicillic acid (PA) is a polyketide mycotoxin produced by several species of Aspergillus and Penicillium. This mycotoxin is toxic in experimental animals and has also been reported to be carcinogenic. The cytotoxicity of penicillic acid was studied in rat albeolar macrophages (AM) in vitro. The effects of penicillic acid on membrane integrity were studied by measuring cell volume changes and /sup 51/Cr release. There was a significant decrease in adenosine triphosphate (ATP) in cell cultures exposed to 1.0 mM penicillic acid for 4 hr. Inhibition of the incorporation of (/sup 3/H)leucine into protein was both dose- and time-dependent and protein synthesis was inhibited significantly after 2 hr exposure to greater than or equal to0.1 mM penicillic acid. RNA synthesis was inhibited to a lesser extent than protein synthesis. There was significant inhibition of phagocytosis after 2 hr exposure at greater than or equal to0.3 mM penicillic acid and the ED/sub 50/ for phagocytosis was 0.09 mM. Thus phagocytosis was more sensitive to the toxic effects of penicillic acid than any other cellular process studied. The data suggest the possibility of a respiratory hazard to agricultural workers exposed to contaminated grain.
- Research Organization:
- National Institute for Occupational Safety and Health, Morgantown, WV
- OSTI ID:
- 7164746
- Journal Information:
- Environ. Res.; (United States), Vol. 38:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MACROPHAGES
PATHOLOGICAL CHANGES
TOXINS
TOXICITY
ASPERGILLUS
ATP
BIOSYNTHESIS
CHROMIUM 51
IN VITRO
INHIBITION
PENICILLIUM
PHAGOCYTOSIS
PROTEINS
RATS
RNA
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FUNGI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHAGOCYTES
PLANTS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SYNTHESIS
TOXIC MATERIALS
VERTEBRATES
550901* - Pathology- Tracer Techniques